论文部分内容阅读
目的:研究地塞米松对白细胞介素IL-13诱导的大鼠鼻黏膜mCLCA3和黏蛋白Muc5ac表达的影响,探讨其对变应性鼻炎黏液分泌的影响。方法:30只SD大鼠随机分成对照组、IL-13组、地塞米松组,用逆转录聚合酶链反应法(RT-PCR)和免疫组化法分别检测鼻黏膜mCLCA3 mRNA和Muc5ac蛋白的表达。结果:对照组大鼠鼻黏膜中mCLCA3mRNA表达为0.000±0.000,IL-13组为0.319±0.121,两组比较差异有统计学意义(P<0.05);地塞米松组为0.144±0.105,IL-13组为0.319±0.121,两组比较差异有统计学意义(P<0.05);对照组大鼠鼻黏膜中Muc5ac蛋白的表达(0.300±0.145)与IL-13组(1.389±0.499)比较,差异有统计学意义(P<0.05);地塞米松组Muc5ac蛋白的表达(0.901±0.390)较IL-13组(1.389±0.499)明显减少(P<0.05)。结论:IL-13能够上调mCLCA3 mRNA和Muc5ac在鼻黏膜的表达,地塞米松可下调mCLCA3 mRNA和黏蛋白Muc5ac表达。
AIM: To investigate the effect of dexamethasone on the expression of mCLCA3 and mucin Muc5ac in the nasal mucosa induced by interleukin-11 (IL-13) in rats and its effect on the mucus secretion of allergic rhinitis. Methods: Thirty SD rats were randomly divided into control group, IL-13 group and dexamethasone group. The expression of mCLCA3 mRNA and Muc5ac protein in nasal mucosa were detected by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry expression. Results: The expression of mCLCA3mRNA in the nasal mucosa of the control group was 0.000 ± 0.000, and that in the IL-13 group was 0.319 ± 0.121, the difference was statistically significant (P <0.05); the dexamethasone group was 0.144 ± 0.105, IL- (P <0.05). Compared with IL-13 group (1.389 ± 0.499), Muc5ac protein expression in nasal mucosa in control group was significantly higher than that in control group (1.389 ± 0.499), the difference was statistically significant (P <0.05). The Muc5ac protein expression in dexamethasone group (0.901 ± 0.390) was significantly lower than that in IL-13 group (1.389 ± 0.499) (P <0.05). Conclusion: IL-13 can upregulate the expression of mCLCA3 mRNA and Muc5ac in nasal mucosa. Dexamethasone can down-regulate mCLCA3 mRNA and Muc5ac expression.