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目的:了解金华市艾滋病筛查实验室检测总体情况,探讨目前影响实验室工作的相关因素和存在的问题,促进全市艾滋病检测筛查实验室的能力建设,提高检测水平。方法:发放质控血清和问卷调查表,对全市艾滋病实验室进行室间质量考评,分析2003~2008年室间质量考评结果,部分实验室进行现场抽查。结果:参加室间质评的实验室逐年增多,检测试剂均为国家批批检合格产品,以第三代国产试剂为主,部分单位使用第四代进口试剂。盲样检测定性结果判断正确,每年均有超过90%的单位对阳性质控标本SI值≤2,说明总体上各级实验室检测能力保持在较高的水平。结论:全市艾滋病检测筛查实验室总体上检测质量较好,能够满足本市艾滋病筛查工作的需要,但质量控制观念和生物安全防护意识还有待提高。
Objective: To understand the overall situation of AIDS screening laboratory in Jinhua City, to explore the current factors affecting the laboratory work and existing problems, to promote the city’s HIV screening laboratory capacity-building, improve the level of detection. Methods: The quality control serum and questionnaires were distributed to the AIDS laboratory in the city to conduct inter-laboratory quality assessment. The results of inter-laboratory quality assessment from 2003 to 2008 were analyzed. Some laboratories conducted spot checks. Results: The laboratories participating in laboratory quality assessment increased year by year. The test reagents were qualified by the state for batch examination and the third generation of domestic reagent was the main reagent. Some units used the imported reagent of the fourth generation. Blind-like test Qualitative results to determine the correct, each year more than 90% of the positive control samples SI value ≤ 2, indicating the overall level of laboratory testing at all levels to maintain a high level. Conclusion: The HIV testing and screening laboratories in the city generally have good testing quality and can meet the needs of HIV / AIDS screening in this Municipality. However, the quality control concepts and awareness of biosafety needs to be improved.