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目的 研究HIV-1相关的等位基因CCR5△32、CCR5m303、CCR2b和SDF1在艾滋病病毒感染人群中的分布,为深入分析这些遗传突变在HIV-1感染、艾滋病发病进程中的作用提供理论依据。方法 收集深圳地区近年来艾滋病病毒感染者的血液标本共91份,提取基因组DNA。经PCR或PCR-RFLP分析,计算遗传突变频率、纯合子和杂合子的构成比例。采用x2检验或t检验进行群体分布的拟合度和差异的显著性分析。结果 在所有被检测的91例HIV-1感染者的个体中,未发现有CCR5△32和CCR5m303的遗传突变,CCR2b-64I和SDF1-3＇A的突变频率较高,分别为21.43％和25.82％。与中国普通汉族人的结果相近。结论 研究结果提示深圳地区的艾滋病病毒感染者本身对HIV-1病原体可能具有较大的遗传易感性。由于他们具有较高的CCR2b-64I和SDF1-3＇A突变频率,后者在艾滋病发病过程中的影响值得进一步研究。
Objective To study the distribution of HIV-1 related alleles CCR5 △ 32, CCR5m303, CCR2b and SDF1 in HIV-infected population and provide a theoretical basis for further analysis of the role of these genetic mutations in HIV-1 infection and AIDS progression. Methods A total of 91 blood samples collected from HIV-infected persons in Shenzhen in recent years were collected and genomic DNA was extracted. The frequencies of genetic mutations, constituent ratios of homozygotes and heterozygotes were calculated by PCR or PCR-RFLP analysis. Significant analysis of the fitness and differences in population distribution using the x2 test or the t-test. Results No genetic mutation in CCR5 △ 32 and CCR5m303 was detected in all the 91 HIV-1 infected individuals. The frequencies of mutations of CCR2b-64I and SDF1-3’A were 21.43% and 25.82 %. Similar to the results of ordinary Han Chinese. Conclusions The findings suggest that people living with HIV in Shenzhen may themselves have a greater genetic susceptibility to HIV-1 pathogens. Because of their high frequency of CCR2b-64I and SDF1-3’A mutations, the impact of the latter in the pathogenesis of AIDS deserves further study.