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研究了猪心肌和马心肌高铁肌红蛋白(pMetMb、hMetMb)被紫外光照射后的荧光光谱变化,紫外光照射时间共设6个梯度,分别是0、5、10、20、30、60min。随着紫外光的照射,MetMb高级结构发生蜕变。利用同步荧光光谱指认了紫外照射对酪氨酸(Tyr)和色氨酸(Trp)的影响,紫外照射0～5min氨基酸残基吸收强度减小;5～30min吸收强度总体保持上升趋势;30～60min吸收强度减小。且指认出MetMb中Trp在该蛋白质肽链中的比例高于Tyr,pMetMb和hMetMb两种荧光光谱谱征变化类似。进一步指认了MetMb的heme-Fe3+荧光谱征变化,照射5min时heme-Fe3+荧光吸收强度降低,10min时荧光吸收强度有所跳跃,20min后持续下降,heme-Fe3+高级结构明显降解或被破坏,可能是MetMb丧失生物活性的又一荧光光谱变化特征。
Fluorescence spectra of porcine myocardium and horse myoglobin (hMetMb) after UV irradiation were studied. The time of UV irradiation was 6 gradients, which were 0, 5, 10, 20, 30 and 60 min. With the UV light irradiation, MetMb advanced structure metamorphosis. The effect of ultraviolet irradiation on tyrosine (Tyr) and tryptophan (Trp) was identified by synchronous fluorescence spectroscopy. The absorption intensity of amino acid residues decreased from 0 to 5 minutes after ultraviolet irradiation. The absorption intensity of 5 ~ 60min absorption intensity decreases. And the recognition that the proportion of Trp in the peptide chain of MetMb is higher than that of Tyr, the changes of fluorescence spectra of pMetMb and hMetMb are similar. The change of heme-Fe3 + fluorescence sign of MetMb was further confirmed. The fluorescence absorption intensity of heme-Fe3 + decreased when irradiated for 5min, the intensity of fluorescence absorption leaped at 10min, decreased continuously after 20min, and the heme-Fe3 + higher structure was obviously degraded or destroyed, Is yet another change in the fluorescence spectrum of the biological activity of MetMb.