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目的:探讨基质金属蛋白酶-9(MMP-9)及基质金属蛋白酶组织抑制物-1(TIMP-1)在卵巢上皮性癌血管生成中的作用。方法:采用免疫组织化学SP法检测45例卵巢上皮性癌组织中MMP-9和TIMP-1的表达,并检测其中微血管密度(MVD,CD34标记)。结果:卵巢癌组织和卵巢良性肿瘤中MMP-9阳性表达率明显高于正常卵巢组织(P<0.01),而TIMP-1未见升高(P>0.05),并且TIMP-1在卵巢癌组织中的阳性表达率显著低于MMP-9(P<0.01)。MMP-9在卵巢癌组织中表达与MVD显著正相关(r=0.286,P<0.05),而TIMP-1与MVD无相关性(r=0.194,P>0.05)。卵巢癌中MMP-9表达高于TIMP-1者,其MVD显著高于MMP-9表达低于TIMP-1者(P<0.05)。结论:MMP-9和TIMP-1表达失衡可能在卵巢上皮性癌血管生成中起重要作用,MMP-9表达增强而TIMP-1表达降低,其血管生成能力可能显著增强,但并非唯一决定因素。检测卵巢上皮性癌中MMP-9和TIMP-1表达对进一步了解卵巢上皮性癌局部血管生成情况有一定应用价值。
Objective: To investigate the role of matrix metalloproteinase-9 (MMP-9) and matrix metalloproteinase-1 (TIMP-1) in angiogenesis of ovarian epithelial carcinoma. Methods: Immunohistochemical SP method was used to detect the expression of MMP-9 and TIMP-1 in 45 cases of epithelial ovarian cancer and to detect the microvessel density (MVD, CD34). Results: The positive rate of MMP-9 in ovarian cancer tissues and ovarian benign tumors was significantly higher than that in normal ovarian tissues (P <0.01), but not in TIMP-1 (P> 0.05) In the positive expression rate was significantly lower than MMP-9 (P <0.01). The expression of MMP-9 in ovarian cancer tissues was positively correlated with MVD (r = 0.286, P <0.05), but there was no correlation between TIMP-1 and MVD (r = 0.194, P> 0.05). The expression of MMP-9 in ovarian cancer was higher than that in TIMP-1, and the MVD in ovarian cancer was significantly higher than that in TIMP-1 (P <0.05). Conclusion: Unbalanced expression of MMP-9 and TIMP-1 may play an important role in the angiogenesis of epithelial ovarian cancer. The expression of MMP-9 is increased and the expression of TIMP-1 is decreased. The angiogenic capacity may be significantly increased but not the only determinant. Detecting the expression of MMP-9 and TIMP-1 in epithelial ovarian cancer has a certain value in further understanding the local angiogenesis of epithelial ovarian cancer.