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以偶氮二异丁腈为引发剂,乙醇为反应介质的单分散聚合法制得了粒径为4.5μm的高度单分散聚苯乙烯(polystyrene)微球。用三氯甲烷和正丙醇将PS微球溶胀,同时吸附荧光素-罗丹明和吖啶橙,制得的微球在荧光显微镜下可观察到被激发出红、绿、蓝等多种颜色的荧光,其发射光谱范围为505到610nm。红外光谱分析表明荧光素被吸收到PS微球内部,而表面没有检测到残余荧光素。从平行试验数据中选出几组最佳条件,采用基因遗传算法优化了溶胀过程中的反应参数,在第六代时得到一组最优反应条件,即10%(wt)的三氯甲烷,15 mmol的荧光素(其中含有33%的吖啶橙),溶胀时间为24h。
Monodisperse polymerization with azobisisobutyronitrile as initiator and ethanol as reaction medium produced highly monodisperse polystyrene microspheres with particle size of 4.5μm. The PS microspheres were swollen with trichloromethane and n-propanol while adsorbing fluorescein-rhodamine and acridine orange. The prepared microspheres were observed under fluorescence microscope to fluoresce in various colors such as red, green and blue Its emission spectrum range is 505 to 610 nm. Infrared spectroscopy showed that fluorescein was absorbed inside the PS microspheres, while no residual fluorescein was detected on the surface. Several groups of optimal conditions were selected from the parallel experimental data. The genetic parameters were optimized by genetic genetic algorithm. At the sixth generation, a group of optimal reaction conditions were obtained: 10% (wt) of chloroform, 15 mmol of fluorescein (which contains 33% of acridine orange), swelling time of 24h.