本文将感染性猪蛔虫卵注入小鼠腹腔,诱导并制备腹腔渗出细胞。玻璃粘附法去掉其中的巨噬细胞,然后用聚蔗糖—复方泛影葡胺进行不连续密度梯度离心,分离嗜酸性粒细胞(Eos)和中粒细胞(Neu),其平均纯度分别为Eos85.5％和Nou86.1％。台盼蓝测两种细胞的活力均在85％以上。文中介绍了一种制备密度梯度的简易装置并对纯化细胞的生物学活性进行了讨论。 Infected Ascaris suum eggs were injected into abdominal cavity of mice to induce and prepare peritoneal exudative cells. The macrophages were removed by the glass adhesion method, and then discontinuous density gradient centrifugation was performed on the Ficoll-Compound diatrizoate to separate the eosinophils (Eos) and the neutrophils (Neu) with the average purity of Eos85 .5% and Nou86.1%. Trypan blue measured the vitality of both cells are more than 85%. In this paper, a simple device for preparing density gradient was introduced and the biological activities of purified cells were discussed.