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In order to specifically detect the localization of thrombus in vivo, we have recently developed two monoclonal antibodies (SZ-58, SZ-63) which can specifically bind to cross-linked fibrin. The binding rates of the two monoclonal antibodies (MoAbs) to human plasma clots in vitro were 46.4±2.3% for ~(125)I-SZ-58, 50.1±1.7% for ~(125)I-SZ-63 and 3.4±1.6% for ~(125)I-SZ-53 (control, MoAb against TM). It was shown that both Sz-58 and SZ-63 posess properties of inhibiting the polymerization of fibrin, and SZ-58 could also inhibit the aggregation of platelets induced by ADP. These characteristics make the two MoAbs suitable in the detection of thrombus in vivo. According to the cross reaction tests, thrombi in the jugular veins and carotid arteries in rabbits were made. After injection of the ~(125)I-labeled MoAbs (100000 cpm/ml of blood), the thrombi and the blood were taken and weighed at various time intervals and radioactivities were measured by an autogamma counter. The ratios of thrombus to blood radi
The order rates of the two monoclonal antibodies (MoAbs) to human plasma clots in vitro were 46.4 ± 2.3% for 125 I-SZ-58, 50.1 ± 1.7% for 125 I-SZ-63 and 3.4 ± 1.6% for 125 I-SZ-53 (control, MoAb against ™). It was shown that both Sz-58 and SZ-63 posess properties of inhibiting the polymerization of fibrin, and SZ-58 could also inhibit the aggregation of platelets induced by ADP. These characteristics make the two MoAbs suitable in the detection of thrombus in vivo. According to the cross reaction tests, thrombi in the jugular veins and carotid arteries in rabbits were made. After injection of 125 I-labeled MoAbs (100000 cpm / ml of blood) the thrombi and the blood were taken and weighed at various time intervals and radioactivities were measured by an autogamma counter. The ratios of thr ombus to blood radi