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目的探讨 ROS影响巨噬细胞凋亡的机制。方法激光扫描共聚焦显微术、流式细胞术和荧光标记技术等。结果 1凋亡巨噬细胞内 NADPH氧化酶活性急剧降低使得胞内 ROS水平快速下降 ;2 ROS清除剂促进地塞米松诱导的巨噬细胞凋亡 ;3PKC促进巨噬细胞凋亡和 ROS急剧减少 ;c AMP抑制巨噬细胞凋亡和 ROS急剧减少。结论 1ROS抑制地塞米松诱导的巨噬细胞凋亡 ;2 PKC、c AMP等因素通过影响地塞米松介导巨噬细胞凋亡时发生的 ROS变化促进或抑制巨噬细胞凋亡。由此可见 ,ROS作为一种巨噬细胞的信使分子和效应分子 ,一方面抑制巨噬细胞自身凋亡 ,一方面执行巨噬细胞介导其它细胞凋亡的作用
Objective To explore the mechanism of ROS affecting macrophage apoptosis. Methods Laser scanning confocal microscopy, flow cytometry, and fluorescent labeling techniques. Results 1 The dramatic decrease of NADPH oxidase activity in apoptotic macrophages caused a rapid decrease in intracellular ROS levels. 2 ROS scavenger promoted dexamethasone-induced macrophage apoptosis; 3PKC promoted macrophage apoptosis and ROS abrupt decrease; cAMP inhibited macrophage apoptosis and dramatically reduced ROS. Conclusion 1ROS can inhibit dexamethasone-induced macrophage apoptosis.2 PKC, cAMP and other factors promote or inhibit macrophage apoptosis by affecting ROS changes induced by dexamethasone-mediated macrophage apoptosis. It can be seen that ROS, as a messenger molecule and effector molecule of macrophages, on the one hand, inhibit macrophage self-apoptosis, and on the other hand, perform macrophages-mediated apoptosis in other cells.