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目的:探讨维生素D受体(FokⅠ)基因多态性与儿童氟斑牙的关系。方法:选取湖南省耒阳市198名8～12岁儿童作为研究对象,采用Dean氏法检查氟斑牙患病情况,多聚酶链反应-限制性片段长度多态性方法分析维生素D受体FokⅠ基因多态性分布。结果:氟斑牙组儿童VDR FokⅠ基因型分布频率为FF 27.1%,Ff 39.6%,ff 33.3%,对照组儿童FF 31.4%,Ff 43.1%,ff 25.5%,两组基因型与等位基因的频率分布差异均无统计学意义(p>0.05)。结论:维生素D受体FokⅠ基因多态性与高氟区儿童氟斑牙发生无明显关系。
Objective: To investigate the relationship between vitamin D receptor (Fok Ⅰ) gene polymorphism and dental fluorosis in children. Methods: A total of 198 children aged 8-12 years in Shenyang were selected as research objects. The prevalence of dental fluorosis was examined by Dean’s method. The FokⅠ gene of vitamin D receptor was analyzed by polymerase chain reaction-restriction fragment length polymorphism Polymorphism distribution. Results: The distribution frequency of VDR FokⅠ genotype in children with dental fluorosis was FF 27.1%, Ff 39.6%, ff 33.3%, FF 31.4%, Ff 43.1%, ff 25.5% respectively in the control group, genotype and allele There was no significant difference in frequency distribution (p> 0.05). Conclusion: There is no significant relationship between Fok Ⅰ gene polymorphism of vitamin D receptor and dental fluorosis in children with high fluoride content.