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目的:观察棉酚在体外对人胃癌细胞株MGC-803细胞增殖、凋亡及AIF表达的影响。方法:MTT法检测细胞增殖、AO/EB双染法和流式细胞仪Annexin VFITC/PI双染法检测细胞凋亡情况,Western blot检测AIF表达情况。结果:棉酚能有效抑制MGC-803细胞增殖,呈一定时效关系。其在24、48、72 h的IC50分别为57.58、53.92、39.10μmol/L。AO/EB双染法和Annexin VFITC/PI双染法检测结果显示棉酚在10、40、80μmol/L作用24 h后能明显促进人胃癌MGC-803细胞凋亡。Western blot结果显示,棉酚使人胃癌MGC-803细胞中AIF在线粒体中表达量减少,细胞核中表达量增加。结论:棉酚可诱导人胃癌MGC-803细胞凋亡。
Objective: To observe the effects of gossypol on the proliferation, apoptosis and AIF expression of human gastric cancer cell line MGC-803 in vitro. Methods: Cell proliferation was detected by MTT assay. Apoptosis was detected by AO / EB double staining and flow cytometry Annexin VFITC / PI double staining. AIF expression was detected by Western blot. Results: Gossypol can effectively inhibit the proliferation of MGC-803 cells in a time-dependent manner. The IC50 at 24, 48 and 72 h were 57.58, 53.92 and 39.10 μmol / L, respectively. The results of AO / EB double staining and Annexin VFITC / PI double staining showed that gossypol could obviously promote the apoptosis of human gastric cancer cell line MGC-803 after treated with 10, 40 and 80μmol / L for 24 h. Western blot results showed that gossypol reduced the expression of AIF in mitochondria and increased the expression of AIF in human gastric cancer cell line MGC-803. Conclusion: Gossypol can induce apoptosis of human gastric cancer cell line MGC-803.