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目的:建立HPLC法测定濒危野生药用植物中华补血草(Limonium sinense(Girard)Kuntze)中异槲皮苷、桑色素、槲皮素、木犀草素和芹菜素的含量,为中华补血草的质量控制提供理论依据。方法:采用高效液相色谱法同时测定中华补血草中异槲皮苷、桑色素、槲皮素、木犀草素和芹菜素的含量。色谱柱:TURNER KromasilTMC18柱(250 mm×4.6 mm,5μm),以甲醇-0.4%磷酸溶液为流动相进行梯度洗脱,流速为1 mL·min-1,检测波长为360 nm,柱温30℃。结果:异槲皮苷、桑色素、槲皮素、木犀草素和芹菜素的质量浓度与峰面积分别在1.00~99.67 mg·L-1(r=0.9994)、0.45~45.42 mg·L-1(r=0.9997)、0.77~76.57 mg·L-1(r=0.9996)、0.43~42.72 mg·L-1(r=0.9997)、0.55~55.39 mg·L-1(r=0.9998)范围内呈良好的线性关系;平均加样回收率(n=6)分别为98.9%、98.6%、98.6%、97.8%、98.7%,RSD为1.6%、1.7%、1.7%、2.2%、1.7%。结论:方法简便可行、重复性好,适合于同时测定中华补血草中异槲皮苷、桑色素、槲皮素、木犀草素和芹菜素的含量,可作为我国中华补血草质量评价的方法之一。
OBJECTIVE: To establish an HPLC method for the determination of isoquercitrin, morin, quercetin, luteolin and apigenin in the endangered wild medicinal plant Limonium sinense (Girard) Kuntze. Control provides a theoretical basis. Methods: Simultaneous determination of isoquercitrin, morin, quercetin, luteolin and apigenin in Limonium sinestium by high performance liquid chromatography. The column was TURNER Kromasil TMMC18 (250 mm × 4.6 mm, 5 μm). The mobile phase was eluted with methanol-0.4% phosphoric acid solution at a flow rate of 1 mL · min-1. The detection wavelength was 360 nm and the column temperature was 30 ℃ . Results: The mass concentration and peak area of isoquercitrin, morin, quercetin, luteolin and apigenin were 1.00 ~ 99.67 mg · L -1 (r = 0.9994) and 0.45 ~ 45.42 mg · L -1 (r = 0.9997), 0.77-76.57 mg · L-1 (r = 0.9996), 0.43-42.72 mg · L-1 (r = 0.9997) and 0.55-55.39 mg · L-1 (r = 0.9998) The average recoveries (n = 6) were 98.9%, 98.6%, 98.6%, 97.8% and 98.7%, respectively. The RSDs were 1.6%, 1.7%, 1.7%, 2.2% and 1.7%, respectively. Conclusion: The method is simple and feasible with good repeatability and suitable for the simultaneous determination of isoquercitrin, morin, quercetin, luteolin and apigenin in Limonium sinense, which can be used as a method for quality assessment of Limonium sinense in China one.