论文部分内容阅读
采用复合PCR扩增 ,6 %变性聚丙烯酰胺凝胶电泳结合银染技术首次对我围新疆哈萨克族 (n =10 2 )和锡拍族 (n=110 )群体进行D16S5 39、D7S82 0、D13S317三个STR位点的调查 ,建立了这两个民族群体遗传学数据库。经统计学检验 ,三个位点的基因型频率分布符合Hardy -Weinberg平衡定律。结果显示 ,哈萨克族群体中观察到 8、7、8种等位片段 ,2 4、2 0、2 3种基因型 ,杂合度为 :0 .80 19- 0 .82 5 9;而锡伯族群体中则观察到 7、8、8种等位片段 ,2 2、2 0、2 1种基因型 ,杂合度为 :0 .8875 0 .92 86。此外 ,比较两个民族的多态性分布 ,发现在D7S82 0位点上无统计学意义 (P >0 .0 5 ) ,而其他两个位点均可见显著性差异P <0 .0 5 )。三个STR位点在两个民族中的综合检验对于民族起源、变迁和民族识别以及民族间亲缘关系研究提供了较高的价值
Using multiplex PCR amplification and 6% denaturing polyacrylamide gel electrophoresis combined with silver staining technology, we conducted the first D16S539, D7S82 0, D13S317 Three STR sites survey established the two ethnic groups genetic database. Statistically, the genotype frequencies of the three loci were in accordance with the Hardy-Weinberg equilibrium law. The results showed that 8,7,8 alleles and 24,2,0,23 genotypes were observed in Kazak population with heterozygosity of 0.80 19- 0.82 59, while Xibe population Among them, 7,8,8 alleles and 2 2,2 0,2 1 genotypes were observed, with the heterozygosity of 0.8875 0 .92 86. In addition, comparing the distribution of polymorphisms in the two ethnic groups, we found no significant difference at the D7S82 locus (P> 0.05), but significant differences at the other two loci (P <0.05) . The combined test of the three STR loci in the two ethnic groups provides a high value for the study of ethnic origin, change and ethnic identity, and ethnicity