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[目的]观察间质流膏对博莱霉素致大鼠肺纤维化PDGF-BB与α-SMA表达影响。[方法]使用随机平行对照方法,将96只雄性Wistar清洁级大鼠编号按随机数字表法分为5组,A对照组(12只)、B模型组(28只)、C间质流膏加地塞米松组(28只)、D间质流膏组(28只)、E地塞米松组(28只)。10%水合氯醛大鼠腹腔注射麻醉,额镜直视下气管插管,气管滴入博来霉素生理盐水0.1mL(5mg/kg),滴注后立即将大鼠直立旋转,使药液在肺内分布均匀,复制肺纤维化模型。A、B组生理盐水腹腔注射0.3ml/d。C组间质流膏灌胃(党参24g,黄芪18g,白术、茯苓各12g,沙参20g,水蛭研末冲6g,蜈蚣研末冲2条,丹参21g,桃仁、川芎、地龙各15g,黄芩12g,白花蛇舌草、薏苡仁各30g,蒲公英24g,浙贝15g,莪术12g,山慈菇21g,甘草12g,连翘、鱼腥草各18g)10mL/kg大鼠体重(生药4.6g/10mL),地塞米松腹腔注射1mg/kg,1次/d。D组间质流膏灌胃(生药4.6g/10mL)10mL/kg大鼠体重,1次/d。E地塞米松腹腔注射1mg/kg,1次/d。连续干预4周(28d)。实验第28天A组脱颈椎处死大鼠3只,其他组分别处死大鼠7只,暴露气管,向大鼠右肺灌注10%甲醛溶液,使胸膜展平后将右肺浸入10%甲醛溶液中固定。常规石蜡包埋,切片(4μm)行HE和免疫组化染色。观测光镜下肺组织病理细胞、肺组织PDGFBB、α-SMA。[结果]B模型组肺泡结构破坏,肺泡壁显著增厚,肺间质纤维化瘢痕形成,毛细血管腔闭塞;C间质流膏加地塞米松组肺组织呈轻中度肺纤维化改变,病变范围局限,肺泡间隔胶原沉积较模型组减少;D间质流膏组与激素组比较肺纤维化程度明显减轻,较正常组略有改变。E地塞米松组肺纤维化改变不明显。肺纤维化程度由轻到重依次为:A组>C组>D组>E组>B组。PDGF-BB值,与A组比较,B组各时间段均明显增高(P<0.05);7d组间比较(P<0.01),D组与E组(P>0.05);14d组间比较(P<0.01),D组与E组(P>0.05);21d组间比较(P<0.01),两两比较均(P<0.05);28d组间比较(P<0.01),两两比较均(P>0.05)。[结论]间质流膏可明显降低博莱霉素致大鼠肺纤维化模型PDGF-BB与α-SMA表达。
[Objective] To observe the effect of interstitial fluid cream on the expression of PDGF-BB and α-SMA in bleomycin-induced pulmonary fibrosis in rats. [Methods] Ninety-six male Wistar clean-grade rats were randomly divided into five groups according to a random number table method, A control group (12), B model group (28), C interstitial fluid cream Dexamethasone group (n = 28), D-mesenchymal paste group (n = 28) and dexamethasone group (n = 28). 10% chloral hydrate rats were anesthetized by intraperitoneal injection and endotracheal intubation was performed under the frontal mirror. Bleomycin physiological saline 0.1 ml (5 mg / kg) was dripped intratracheally and the rats were erected immediately after instillation, In the lung evenly distributed, replicate lung fibrosis model. A, B group saline intraperitoneal injection of 0.3ml / d. C group of interstitial paste orally (Codonopsis 24g, Astragalus 18g, Atractylodes, Poria each 12g, Radix 20g, Leech Yanmo Chong 6g, centipede Yan Mo Chong 2, Salvia 21g, peach kernel, Chuanxiong, earthworm each 15g, Scutellariae 12g, Hedyotis diffusa, Coix 30g each, dandelion 24g, Zhejiang shellfish 15g, Curcuma 12g, Capsicum 21g, licorice 12g, forsythia, Houttuynia 18g) 10mL / kg rat body weight / 10mL), dexamethasone intraperitoneal injection of 1mg / kg, 1 / d. D group between the mass flow cream orally (crude drug 4.6g / 10mL) 10mL / kg body weight, 1 / d. E dexamethasone intraperitoneal injection of 1mg / kg, 1 time / d. Continuous intervention for 4 weeks (28d). On the 28th day of experiment, three rats in group A were killed by cervical debridement. In the other groups, 7 rats were killed, trachea were exposed, 10% formalin was infused into the right lung of rats, the right lung was immersed in 10% formaldehyde solution Fixed. Conventional paraffin embedded, sectioned (4μm) HE and immunohistochemical staining. Under the light microscope, pathological lung cells, lung tissue PDGFBB, α-SMA. [Results] Alveolar structure destruction, alveolar wall thickening, interstitial fibrosis scar formation and capillary cavity occlusion were observed in model group B. The lung tissue of C-mesenchymal fluid plus dexamethasone group showed mild to moderate pulmonary fibrosis, Range limitations, alveolar septum collagen deposition decreased compared with the model group; D interstitial fluid cream group and the hormone group compared to the degree of pulmonary fibrosis was significantly reduced compared with the normal group slightly changed. E dexamethasone group, pulmonary fibrosis did not change significantly. The degree of pulmonary fibrosis from light to heavy were as follows: A group> C group> D group> E group> B group. PDGF-BB in group B were significantly higher than those in group A (P <0.05); Group D was significantly higher than group E (P <0.05); Group D was significantly higher than group E (P <0.01), D group and E group (P> 0.05); 21d group comparison (P <0.01), any pairwise comparison (P <0.05); 28d group comparison (P> 0.05). [Conclusion] Interstitial fluid cream can significantly reduce the expression of PDGF-BB and α-SMA induced by bleomycin in rat pulmonary fibrosis model.