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目的 :探讨代谢酶CYP1A1和GSTM1等位基因型对烟酒诱发人体内淋巴细胞微核的影响。方法 :分别应用等位基因特异性(AS)和多重差别 (MD) -PCR检测CYP1A1和GSTM1的等位基因型 ,应用末梢血微核法检测体内淋巴细胞微核。结果 :与无吸烟史健康人群的淋巴细胞平均微核率 (MNF 0 .2 4‰ )相比 ,吸烟组MNF显著增加 (0 6 5‰ ,P <0 .0 5 ) ,重度饮酒可增强这一效应 (0 .89‰ ,P <0 .0 1) :与无吸烟史的非易感联合基因型 (CYP1A1I1e/I1e·GSTM1+(+/ +和 +/ 0 ) ,CYP1A1I1e/I1e·GSTM10 / 0和CYP1A1I1e/Val·GSTM1+)个体的MNF(0 .42‰ )相比 ,吸烟使GSTM10 / 0基因型个体的MNF显著上升 (0 .75‰ ,P <0 .0 5 ) ,并可使CYP1A1Val/Val基因型和易感联合基因型 (CYP1A1I1e/Val·GSTM10 / 0 ,CYP1A1Val/Val·GSTM1+和CYP1A1Val/Val·GSTM10 / 0 )个体的MNF均上升约 1倍 (0 .83‰和 0 .85‰ ,0 10 >P >0 .0 5 )。结论 :吸烟诱发体内淋巴细胞MNF显著增加 ,重度饮酒增强这一效应。烟酒诱发微核形成与个体CYP1A1和GSTM1的遗传多态性密切相关。
Objective: To investigate the effects of CYP1A1 and GSTM1 alleles on micronuclei in lymphocytes induced by smoking and drinking. Methods: The genotypes of CYP1A1 and GSTM1 were detected by allele specific (AS) and multiplex differential (PCR) -PCR respectively. The micronuclei of lymphocytes were detected by peripheral blood micronucleus test. Results: MNF was significantly increased in smoking group (0.665 ‰, P <0.05) compared with MNF (0.24 ‰) in healthy smokers, and heavy drinking increased One effect (0.89 ‰, P <0.01) was associated with non-susceptible genotypes (CYP1A1I1e / I1e · GSTM1 + (+ / + and + / 0) and CYP1A1I1e / I1e · GSTM10 / 0 Compared with MNF (0.42 ‰) in CYP1A1I1e / Val · GSTM1 + individuals, smoking significantly increased MNF in GSTM10 / 0 genotype (0.75 ‰, P <0.05), and CYP1A1Val / The MNFs of Val genotypes and susceptible genotypes (CYP1A1I1e / Val · GSTM10 / 0, CYP1A1Val / Val · GSTM1 + and CYP1A1Val / Val · GSTM10 / 0) all increased by about 1 times (0.83 ‰ and 0.85 ‰ , 0 10> P> 0. 0 5). Conclusion: Smoking induced MNF in lymphocytes significantly increased, severe alcohol consumption increased this effect. Tobacco and alcohol induced micronucleus formation is closely related to the genetic polymorphisms of CYP1A1 and GSTM1.