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为了解国内羊肺炎支原体的流行情况,本研究应用正向间接血凝试验(IHA)对2014年~2015年间采自我国12个省44个市、县的3 402份未免疫血清样品进行了绵羊肺炎支原体(MO)、山羊支原体山羊肺炎亚种(Mccp)抗体的检测。结果显示:MO抗体阳性率为31.92%(1 086/3 402),其中阳性率最高的省份为内蒙古76.8%(636/828),其次是河北50%(60/120)、吉林21.99%(93/423);Mccp的抗体阳性率为10.67%(363/3 402),其中阳性率最高省份为河南35.14%(39/111),其次为河北27.5%(33/120)、山东22.5%(27/120);另有来自河北、辽宁、吉林、海南、黑龙江、内蒙古和安徽7省份的少量血清样品同时检测出MO和Mccp两种抗体,表明该病的流行存在不同菌株混合感染的情况,这在河北地区最为严重(15%)。分析表明,羊支原体肺炎在我国大部分地区流行相当严重,尤其在河北、河南和山东等集约化舍饲养殖发展的地区。绵羊的抗体阳性率明显高于山羊,表明MO在致病性和感染性方面相对Mccp更强。本研究为评估羊支原体肺炎在我国的流行状况以及制定有效防制措施提供了实验依据。
In order to understand the prevalence of Mycoplasma hyopneumoniae in China, 3 402 non-immune serum samples collected from 44 cities and counties in 12 provinces of China from 2014 to 2015 were analyzed by indirect hemagglutination test (IHA) Mycoplasma pneumoniae (MO), Mycoplasma goat pneumonia (Mccp) antibody detection. The results showed that the positive rate of MO antibody was 31.92% (1 086/3 402), the highest positive rate was 76.8% (636/828) in Inner Mongolia, followed by Hebei 50% (60/120), Jilin 21.99% (93% / 423). The positive rate of Mccp was 10.67% (363/3402). The highest positive rate was 35.14% (39/111) in Henan, followed by 27.5% (33/120) in Hebei and 22.5% (Shandong) / 120). In addition, a small amount of serum samples from Hebei, Liaoning, Jilin, Hainan, Heilongjiang, Inner Mongolia and Anhui Provinces detected MO and Mccp antibodies at the same time, indicating that the prevalence of this disease was mixed infection of different strains, which The most serious in Hebei (15%). Analysis shows that Mycoplasma pneumoniae pneumonia in most parts of China epidemic quite serious, especially in Hebei, Henan and Shandong intensive care breeding areas. The positive rate of antibodies in sheep was significantly higher than that of goats, indicating that MO is more potent in Mcpath than pathogenicity and infectivity. This study provides an experimental basis for assessing the prevalence of Mycoplasma hominis pneumonia in our country and formulating effective prevention and control measures.