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目的:证实胶质源性神经营养因子(glialcellline-derivedneurotrophicfactor,GDNF)对脊髓前角运动神经元有保护作用。方法:切断SD大鼠一侧坐骨神经建立脊髓前角运动神经元损伤模型,借助单盲端硅胶管系统在损伤神经局部给予GDNF,术后不同时间分别取L5脊髓切片,利用酶组织化学染色方法显示胆碱酯酶和酸性磷酸酶(acidphosphatase,ACP)活性并进行图像分析。结果:坐骨神经切断后第4,9,16及21d,对照组和GDNF组胆碱酯酶活性分别为21.53±1.54和23.67±1.08(P<0.05),19.82±1.35和22.87±1.04(P<0.01),22.55±1.20和25.25±1.13(P<0.01),25.52±1.43和28.92±1.37(P<0.01);对照组和GDNF组ACP活性(平均灰度)分别为37.49±1.39和35.40±1.18(P<0.05),40.94±1.38和38.43±1.31(P<0.05),22.55±1.20和25.25±1.13(P<0.05),37.15±1.52和34.68±1.43(P<0.05)结论:GDNF对损伤的脊髓前角运动神经元有保护作用。
Objective: To investigate the protective effect of glial cell line-derived neurotrophic factor (GDNF) on motor neurons in anterior horn of spinal cord. Methods: The spinal cord anterior horn motor neuron injury model was established by cutting off the sciatic nerve on one side of SD rats. GDNF was given locally to the injured nerve via a single-ended silicone tube system. L5 spinal cord slices were taken at different time points after operation, and enzymatic histochemical staining was used Cholinesterase and acid phosphatase (ACP) activity and image analysis. Results: The cholinesterase activities of control group and GDNF group were 21.53 ± 1.54 and 23.67 ± 1.08 (P <0.05), 19.82 ± 1.35 and 22.87 ± 1.04 (P <0.01) on the 4th, 9th, 16th and 21th day after sciatic nerve was cut off ), 22.55 ± 1.20 and 25.25 ± 1.13 (P <0.01), 25.52 ± 1.43 and 28.92 ± 1.37, respectively (P <0.01). The ACP activities in control group and GDNF group were 37.49 ± 1.39 and 35.40 ± 1.18 P <0.05), 40.94 ± 1.38 and 38.43 ± 1.31 (P <0.05), 22.55 ± 1.20 and 25.25 ± 1.13 (P <0.05), 37.15 ± 1.52 and 34.68 ± 1.43 (P <0.05) Anterior horn motor neurons have a protective effect.