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哺乳类细胞在体内或体外培养条件下的生长依赖多肽生长刺激因子(如 EGF,FGF,PDGF)的存在,其细胞膜上有特异的膜受体将增殖信号传导到细胞核,与感受态(competence)基因的表达有关,其中二个基因与原癌基因 C-fos 和 C-myc 相关。这些基因的表达在一定程度上控制细胞的生长状态。Takehara 等近来研究了 TGF-β对血管内皮细胞生长的作用,发现 TGF-β是血管内皮细胞增殖的强效抑制物,并首次报道 TGF-β通过降低在 G_0/G_1期发挥作用的感受态基因的表达来抑制内皮细胞的生长,这种抑制作用与细胞表面的 EGF 高亲和力受体数量变化有关。TGF-β可抑制人脐静脉、牛主动脉、大鼠心脏和大鼠心脏血管内皮等不同来源的内皮细胞的生长。预先1h 用放线菌酮处理一株克隆的大鼠心脏内皮细胞(RHECs),加入 TGF-β后仍很快观察到生长抑制作用,这表明 TGF-β作用不需蛋白质合成的参与。
The growth of mammalian cells in vivo or in vitro culture depends on the presence of polypeptide growth stimulating factors (such as EGF, FGF, PDGF), the membrane-specific membrane receptors on the proliferation signal transduction to the nucleus, and competence (competence) Gene expression, in which two genes associated with proto-oncogene C-fos and C-myc. The expression of these genes to a certain extent control the cell growth state. Recently, Takehara et al. Studied the effect of TGF-β on the growth of vascular endothelial cells and found that TGF-β is a potent inhibitor of vascular endothelial cell proliferation. It was also reported for the first time that TGF-β reduced the expression of competent genes in G_0 / G_1 phase Of the inhibition of endothelial cell growth, this inhibitory effect and cell surface EGF high affinity receptor number changes. TGF-β can inhibit the growth of endothelial cells of different origins, such as human umbilical vein, bovine aorta, rat heart and rat cardiovascular endothelium. One clone of rat cardiac endothelial cells (RHECs) was treated with cycloheximide at 1 h before the growth inhibition was observed soon after the addition of TGF-β, indicating that TGF-β does not require protein synthesis.