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本文比较了犬恶丝虫在3种培养液中的发育情况。犬恶丝虫的感染期幼虫来自阳性埃及伊蚊,处理后接种于下列3种培养液中:(A)含10%人AB型血清,采用HEPES进行缓冲的RPMI 1640。(B)含有机酸(100ml内含失水苹果酸67mg、琥珀酸6mg和α-酮戊二酸37mg、糖(100ml内含葡萄糖70mg和蔗糖2668mg)和10%胎牛血清的TC-199培养液(C)含10%胎牛血清的Grace昆虫培养液。血清在加热灭活后加入培养液。培养液中加入苄青霉素和链霉素并调节pH值为7.2～7.4。以10～12条感染期幼虫培养于盛
This article compares the development of Dirofilaria in three kinds of culture broth. Infectious larvae of the filariasis were from positive Aedes aegypti and were inoculated into the following three cultures after treatment: (A) RPMI 1640 with 10% human type AB serum and buffered with HEPES. (B) TC-199 containing organic acids (100 ml containing 67 mg of dehydrated malic acid, 6 mg of succinic acid and 37 mg of α-ketoglutaric acid, sugar (100 mg glucose 70 mg and 2668 mg glucose) and 10% fetal bovine serum (C) Grace insect culture medium containing 10% fetal bovine serum Serum was added to the culture medium after heat inactivation, and benzyl ampicillin and streptomycin were added to the culture medium to adjust the pH value to 7.2 to 7.4. Infection larval culture in the Sheng