HEPES相关论文
选择10mol/L、20mol/L、30mol/L、40mol/L、50mol/L浓度的HEPES溶液，研究其对BHK-21细胞的影响。结果表明，随着HEPES溶液浓度的增大，......
背景：空间站是空间生物学重要的研究平台,而空间站难以提供细胞培养所需要的CO2 环境,并且需要可控且自动化的细胞培养方法,故建立......
利用狂犬病毒CTN-181株和CTN-BNK株研究感染滴度滴定法发现用HEPES能诱导增强狂犬病毒对感染细胞的致病变作用，当病毒感染细胞后在3......
由3-甲酰基-9-乙基咔唑和吡啶2-甲酰肼缩合制备了一种基于咔唑的结构简单的探针分子1。探针1在CH3CN-H2O(9/1,v/v,HEPES 0.02 mol......
Protein tyrosine phosphatase 1B (PTP1B) has received considerable attention from the drug industry as a potential treatm......
通过罗丹明B与乙二胺反应生成的中间体合成了一例可以对铜离子进行比色检测的探针R-Cu.R-Cu可以实现对在HEPES(5 mmol/L;pH 7.4)溶......
A water-soluble, polythiophene-based colorimetric sensor was designed for selective and sensitive detection of carbenici......
A colorimetric and ratiometric fluorescent chemosensor based on furan-pyrene for selective and sensi
A new pyrene derivative BF bearing a furan group was synthesized via a one-step reaction as a colorimetric and ratiometr......
A rhodamine derivative for Hg~(2+) -selective colorimetric and fluorescent sensing and its applicati
A rhodamine-based sensor has been developed for the detection of mercuric ions. The colorimetric and fluorescence respon......
以9-氨基咔唑与2-羟基-1-萘甲醛为原料,设计合成了一种新型咔唑类席夫碱化合物。通过核磁共振光谱、质谱、红外光谱和元素分析对其......
采用4-羟乙基哌嗪乙磺酸钠-Na OH缓冲溶液(HEPES)为还原剂和稳定剂,制备了复杂三维结构的金纳米花(Au NF)溶胶,采用表面等离子体吸......
以1,8-二乙炔基芘和8-氨基喹啉为原料,通过click反应合成了一种双取代芘荧光探针L,表征了其结构.探针L可以在THF/H_2O(V∶V=1∶1,H......
设计合成基于苯并噻唑Zn~(2+)荧光增强型探针BHP,在HEPES缓冲液中测其对Zn~(2+)识别性能。实验结果表明,BHP对Zn~(2+)有较高的选择......
以2-(2-氨基苯基)-1-苯基-1H-菲并[9,10-d]咪唑和对苯二甲醛为原料,合成了以Schiff碱链接的双菲并咪唑衍生物(BPII),并采用红外和......
Monitoring mitochondrial derived copper(Ⅱ) in live cells is highly demanded, but accurately detecting is unmet due to t......
设计合成了一个基于双吡唑啉结构的双功能荧光探针1,1’-(5,5’-(6,6’-二羟基-5,5’-二甲氧基联苯-3,3’-二基)双(3-(2-羟基苯基)......
以罗丹明B、水合肼及2＇-羟基苯乙酮等廉价化合物为原料,经过简单的缩合反应得到可识别Hg^2＋的荧光探针L,其结构经1HNMR、13CNMR以及H......
Effect of simulated ischemia-reperfusion on I_f in sinoatrial node cells and the intervention of KAT
Objective: To study the effect of simulated ischemia-reperfusion (I/R) on If of sinoatrial node (SAN) cells and the inte......
Atrial fibrillation (AF) is the most common sustained arrhythmia encountered in clinical practice.~1 Its incidence incr......
Background Atrial fibrillation (AF) is the most common supraventricular arrhythmia in clinical practice. Chronic atrial ......
To study the effect of of lidocaine and amiodarone on the transmural heterogeneity of ventricular repolarization in isol......
Peroxisome proliferator-activated receptor-γ (PPAR-γ) is a transcription factor belonging to the nuclear receptor supe......
Age-related changes in dispersion of atrial effective refractory period and its ionic mechanism in c
Atrial fibrillation(AF)is the most common cardiacarrhythmia in man,and epidemiological study hasshown the close relatio......
Effects of Spinach Powder Fat-Soluble Extract on Proliferation of Human Gastric Adenocarcinoma Cells
Four kinds of assays were used to study the effect of a fat-soluble extract of spinach powder (SPFE) on the proliferatio......
α-LTX and α-LTX~(N4C) induce [Ca~(2+)]i elevation through different mechanisms in pancreatic β cells
α-latrotoxin (α-LTX), a neurotoxin from black-widow spider, causes vesicles release in pre- synapse of nerve terminal ......
5.8细胞毒性试验(分子滤过法)5.8.1.目的:设计本试验是用来评价试验材料的细胞毒性.5.8.2.培养基5.8.2.1.生长培养液Eagle’s MEM:......
The composite material of poly-(L-lactide) (PLLA) and carbon nanotubes (CNTs) were prepared. Itssurface morphologies and......
Gold nanoparticles with different morphologies, such as spindle, octahedron, and decahedron were obtained by using diffe......
The effect of nitric oxide donor sodium nitroprusside (SNP) on resting membrane potential (Em) and potassium currents o......
应用超薄层聚丙烯酰胺凝胶等电聚焦电泳对武汉地区198例成人精液黄递酶Ⅲ(DIA_3)作了分型。观察到4种表现型,基因频率分别为DIA_3~......
Objective To investigate changes in the delayed rectifier K + channel (Kv) function and the regulation of Kv by the pro......
本文报道了用含嗜碱性细胞多的细胞悬液对12例棘球蚴病人及68例对照者的嗜碱性细胞脱粒反应的结果。12例病人中有10例手术证实肝......
Brain is very susceptible to hypo-glucose and hypoxia induced by ischemia/reperfusion and significant neuronal injuries ......
Gamma-aminobutyric acid A receptor gamma 2 subunit following Mg-free-induced seizures in cultured de
BACKGROUND:Studies have demonstrated that in vitro cultured cortical neurons from embryonic rats can produce spontaneous......
作者测试了从巴布亚-新几内亚疟区居民血清中分离出的免疫球蛋白对恶性疟原虫体外生长的作用,检测了对有抑制作用的免疫球蛋白敏......
用浓度自12.5～3200μM成倍递增的氯喹、奎宁或甲氟喹水溶液,按每井25μl加入培养板各井内,井径为16mm。干燥后置于15～25℃暗室备用......
MRC-9细胞是1974年得自一15周龄女性胎儿的肺组织,经胰酶消化、连续传代培育的成纤维细胞。该胎儿的母亲14岁,本人及家族无异常病......
本文比较了犬恶丝虫在3种培养液中的发育情况。犬恶丝虫的感染期幼虫来自阳性埃及伊蚊,处理后接种于下列3种培养液中:(A)含10%人A......
体外测定抗体形成细胞(PFC)的影响因素极多,本文主要比较不同批号胎牛血清和绵羊红细胞、血清灭活与否、培养基种类以及培基中是......
HEPES(N-2-羟乙基哌嗪-W′-2-乙磺胺)是一个常用缓冲剂,通常认为除稳定pH 作用外,无其他药理活性。但最近Norris 等证明它可能具......
羊水细胞培养是产前诊断染色体病和先天性代谢病的重要手段,成功而有效的分析取决于羊水细胞培养的质量。参考1983年Chettur提出......
Trager蜡烛缸培养恶性疟原虫的方法,每天需换培养液一次。现利用蜡烛缸方法,通过降低虫血悬液中的红细胞压积和起始原虫率来延长......
研究媒介昆虫如何抗感染有助于制定更为成功的防制策略。本文报道了埃及伊蚊对彭亨丝虫感染免疫反应的实验结果。1.免疫血淋巴对......
Cytosolic free Ca<sup>2-</sup>([Ca<sup>2+</sup>]i)was measured in dissociated cerebral cells isolated from fetal rats wi......
Effects of arsenic trioxide on voltage-dependent potassium channels and on cell proliferation of hum
Arsenic trioxide(ATO)can induce cellular apoptosisand inhibit the activities of multiple myeloma(MM)cells in vitro,1 bu......
