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目的观察叉状头/翅膀状螺旋转录因子(Foxp3)在BXSB狼疮小鼠肝脏的表达,探索调节性T细胞在狼疮肝损害中的可能作用机制。方法以8周龄正常C57BL/6雄性小鼠作对照,用免疫组织化学及实时PCR方法,检测Foxp3在8周、16周龄雄性BXSB小鼠肝脏的表达。结果正常组C57BL/6小鼠肝组织Foxp3阳性细胞呈深棕黄色,位于肝小叶的肝血窦和(或)窦周隙内,且分布较均匀;8周龄及16周龄BXSB小鼠肝组织内Foxp3染色呈浅棕黄色,明显弱于正常鼠,且分布在界板周边较明显。8周、16周BXSB组小鼠肝组织内Foxp3 mRNA表达水平(0.30±0.04、0.18±0.03)较正常对照组(1.08±0.08)明显降低,16周BXSB组的Foxp3 mRNA表达水平较8周BXSB组明显降低,差异均有统计学意义(P<0.01)。结论BXSB小鼠肝损害的发病机制可能与调节性T细胞的Foxp3分布和表达量下调有关。
Objective To investigate the expression of Foxp3 in the livers of BXSB lupus mice and explore the possible mechanism of regulatory T cells in lupus liver damage. Methods Eight-week-old male C57BL / 6 mice were used as controls. Immunohistochemistry and real-time PCR were used to detect the expression of Foxp3 in the liver of male BXSB mice aged 8 weeks and 16 weeks. Results Foxp3 positive cells in normal liver tissue of C57BL / 6 mice were dark brown, located in hepatic sinusoids and / or sinus space of hepatic lobules, and distributed evenly. BXSB mouse liver of 8 weeks old and 16 weeks old Tissue Foxp3 staining was light brown yellow, significantly weaker than normal mice, and distribution in the border around the more obvious. The expression of Foxp3 mRNA in BXSB group was significantly lower than that in normal control group (0.30 ± 0.04,0.18 ± 0.03) at 16 weeks (P <0.01), and the expression level of Foxp3 mRNA in BXSB group was significantly lower than that of BXSB group Group was significantly lower, the difference was statistically significant (P <0.01). Conclusion The pathogenesis of liver damage in BXSB mice may be related to the Foxp3 distribution and down-regulation of regulatory T cells.