论文部分内容阅读
自交不亲和是显花植物的一种重要生殖生理现象,为探明中国梨的自交不亲和特性,对‘锦香’(Pyrus bretschneideri cv. Jinxiang)和‘鹅酥’(Pyrus bretschneideri cv. Esu)2个中国梨品种进行了基因组PCR特异扩增、S基因序列分析及田间杂交授粉试验。结果确定它们各含1个新S-RNA酶基因,分别命名为S37-和S38-RNase,GenBank序列号为DQ839238和DQ839239。生物信息学分析结果表明,S37-和S38-RNA酶的推导氨基酸序列与S1-至S36-RNA酶36个梨S基因具有相同的、高度保守的C1和C2区,但其高变区与S1-至S36-RNA酶差异较大,其中与S15的差异最小,只有3个氨基酸不同。在推导的氨基酸水平上,S37与S38有96%的序列相似性,但两者与S15的相似性更高,皆为98%,与S32的相似性最低,都只有63%;S37和S38的内含子较大,分别为786bp和723bp,与S15的777bp大小接近。最后,经分析验证确定‘锦香’和‘鹅酥’的S基因型分别为S34S37和S15S38。
Self-incompatibility is an important reproductive physiology phenomenon of flowering plants. In order to probe the self-incompatibility of pear in China, the effects of Pyrus bretschneideri cv. Jinxiang and Pyrus bretschneideri cv. Esu) Two Chinese pear cultivars were subjected to genomic PCR-specific amplification, S gene sequence analysis and field cross pollination experiments. As a result, each of them contained one new S-RNase gene, respectively named as S37- and S38-RNase, and GenBank Nos. DQ839238 and DQ839239. Bioinformatics analysis showed that the deduced amino acid sequences of S37- and S38-RNase shared the same highly conserved C1 and C2 regions as those of 36 pear S genes of S1- to S36-RNase, - to S36-RNase differences are larger, with the smallest difference with the S15, only three different amino acids. At the deduced amino acid level, S37 and S38 had a 96% sequence similarity, but their similarity to S15 was higher at 98% and the similarity with S32 was only 63% Introns are larger, 786 bp and 723 bp, respectively, which are close to the 777 bp size of S15. Finally, the S genotypes of ’Jinxiang’ and ’Gooseberry’ were confirmed as S34S37 and S15S38 respectively by analysis and verification.