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目的 应用一种能快速、准确识别胃癌标记染色体来源的技术 ,以提高对胃癌细胞复杂染色体畸变辨认的能力。方法 采用改良的 G显带染色体标本脱色后进行荧光原位杂交 (fluorescence in situhybridization,FISH) ,分别对胃癌细胞系 SGC- 790 1的两条标记染色体 (M1和 M2 )和一例原发性胃癌的标记染色体 (M3)进行分析。结果 显示了 M1、M2和 M3有复杂的染色体结构畸变 :del(7) (p15 ) / del(7)(q2 2 ) ;t(1;3) (p11;q11)和 del(7) (q32 )。结论 该方法具有信号强、背景低和重复性好等优点 ,在识别胃癌染色体复杂结构改变中具有重要的作用。
Objective To apply a technique that can quickly and accurately identify the origin of gastric cancer marker chromosomes to improve the ability to identify complex chromosome aberrations in gastric cancer cells. Methods After the decolorization of the modified G-banding chromosomal specimens, fluorescence in situ hybridization (FISH) was performed on two marker chromosomes (M1 and M2) of gastric cancer cell line SGC-790 1 and one case of primary gastric cancer. Marker chromosomes (M3) were analyzed. The results show that M1, M2, and M3 have complex chromosomal structural aberrations: del(7)(p15)/del(7)(q2 2); t(1;3) (p11;q11) and del(7) (q32 ). Conclusion This method has the advantages of strong signal, low background and good repeatability. It plays an important role in identifying the complex structural changes of chromosomes in gastric cancer.