研究了冷冻蚀刻电镜技术原位观测合成的纳米分散系的制样步骤、制样方法 ,并利用冷冻蚀刻电镜技术原位观测了四种纳米分散系中分散相的粒度、粒度分布和聚集状态 .研究结果表明 :蚀刻时间对电镜照片图像质量有较大影响 ,蚀刻时间应根据分散系中分散相含量、粒度大小来选 ;冷冻蚀刻电镜用于原位观测非水纳米分散系 ,具有准确、直观、清晰、立体的特点 ,并且可同时采集多种信息 . In this paper, the preparation steps and sample preparation methods of in-situ observation of the synthesized nanodispersed systems were studied by freeze-etching electron microscopy. The particle size, particle size distribution and aggregation state of the dispersed phases in the four nanodisperse systems were observed in situ by means of freeze-etching electron microscopy. The results show that the etching time has great influence on the image quality of the electron micrographs. The etching time should be based on the dispersed phase content and particle size in the dispersion system. The cryo-etching electron microscope is used to observe the non-aqueous nanodispersion system in situ with accurate and visual , Clear, three-dimensional characteristics, and can capture a variety of information at the same time.