当前用于旋毛虫病诊断的抗原是直接从培养基中提取的排泄/分泌(ES)抗原,含120000条左右幼虫的75ml培养基中仅提取约1mg的抗原。本文采用旋转两性电解质柱作等电聚焦,直接从肌期幼虫粗抗原中提取ES抗原,并就其在诊断旋毛虫病的特异性和敏感性与体外培养肌期幼虫的ES抗原进行了比较。从猪体内分离旋毛虫,并在1CR小鼠和 Current antigens used for the diagnosis of trichinosis are excretory / secretory (ES) antigens extracted directly from the medium, and only about 1 mg of antigen is extracted from 75 ml of media containing 120,000 larvae. In this paper, the rotating ampholyte column was used for isoelectric focusing to extract ES antigen directly from crude muscle larvae, and its specificity and sensitivity in diagnosis of trichinosis were compared with those of in vitro cultured muscle larvae. Trichinella isolated from pigs and in 1CR mice