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目的为了研究庚型肝炎病毒（ＨＧＶ）在我国的感染状况。方法根据已发表的ＨＧＶ的５’端非编码区（５’－ＵＴＲ区）及螺旋酶区（ＮＳ３区）两段高度保守的基因序列分别设计两套引物，用逆转录－套式聚合酶链式反应（ＲＴ－ｎｅｓｔｅｄＰＣＲ）检测ＨＧＶＲＮＡ。结果从北京、秦皇岛、河南等地采集各种肝病患者及职业献血员血清３５４份，ＨＧＶＲＮＡ阳性７９份，阳性率为２２．３％。其中已确定的临床型肝炎／肝病患者２５４例，ＨＧＶＲＮＡ阳性者为５０例，阳性率为１９．６％。原因不明的或非甲～戊型肝炎患者４３例，ＨＧＶＲＮＡ阳性者为１３例，阳性率为３０．２％。丙型肝炎阳性的职业献血员５７例，ＨＧＶＲＮＡ阳性者为１６例，阳性率为３０．２％。结论提示ＨＧＶ感染在我国多种人群中普遍存在，它不仅可能是引起非甲～戊型肝炎的重要病原之一，也可能是引起输血后肝炎的病原因子
Objective To study the status of hepatitis G virus (HGV) in China. Methods According to the published highly conserved sequences of 5 ’untranslated region (5’-UTR) and helicase (NS3) in HGV, two sets of primers were designed respectively. Two sets of primers were designed by reverse transcription-nested polymerase chain reaction H-RNA was detected by RT-nested PCR. Results A total of 354 serum samples of various liver diseases and professional blood donors were collected from Beijing, Qinhuangdao and Henan Provinces, and 79 were HGV RNA positive. The positive rate was 22.3%. Among them, 254 cases of clinical hepatitis / liver disease have been identified, 50 cases were positive for HGVRNA, the positive rate was 19.6%. In 43 patients with unexplained or non-hepatitis A-E hepatitis, 13 were HGV RNA positive and the positive rate was 30.2%. 57 cases of hepatitis C positive professional blood donors, HGVRNA positive were 16 cases, the positive rate was 30.2%. Conclusions HGV infection is widespread in many populations in China, it may not only be one of the important pathogens causing non-hepatitis E-hepatitis E, but also may be the causative agent of post-transfusion hepatitis