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Aim:To investigate the methylation status of the deleted in azoospermia 1(DAZ1)gene promoter region in differentcell types.Methods:Using CpG island Searcher software,a CpG island was found in the promoter region of theDAZ1 gene.The methylation status of this region was analyzed in sperm and leukocytes by bisulfited sequencing.Results:The methylation status of the CpG island in the DAZ1 gene promoter region differed in leukocytes andsperm:it was methylated in leukocytes,but unmethylated in sperm.Conclusion:A differentially methylated region ofthe DAZ1 gene exists in spermatic and somatic cells,suggesting that methylation of this region may regulate DAZ1gene expression in different tissues.(Asian J Androl 2006 Jan;8:61-67)
Aim: To investigate the methylation status of the deleted in azoospermia 1 (DAZ1) gene promoter region in differentcell types. Methods: Using CpG island Searcher software, a CpG island was found in the promoter region of the DAZ1 gene.The methylation status of this region was analyzed in sperm and leukocytes by bisulfited sequencing. Results: The methylation status of the CpG island in the DAZ1 gene promoter region differed in leukocytes and sperm: it was methylated in leukocytes, but unmethylated in sperm. Confc: A differentially methylated region of the DAZ1 gene suggesting in methylation of this region may regulate DAZ1 gene expression in different tissues. (Asian J Androl 2006 Jan; 8: 61-67)