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研究了２１１Ａｔ通过二乙撑三胺五乙酸（ＤＴＰＡ）酸酐标记人免疫球蛋白Ｇ（ＩｇＧ）的方法。合成ＤＴＰＡ酸酐后与人ＩｇＧ反应，制得ＤＴＰＡ－ＩｇＧ。Ｎａ２１１Ａｔ溶液与分离纯化后的ＤＴＰＡ－ＩｇＧ在室温下反应３０ｍｉｎ，经ＳｅｐｈａｄｅｘＧ５０柱分离纯化，得２１１Ａｔ－ＤＴＰＡ－ＩｇＧ的０．１ｍｏｌ／ＬＰＢＳ淋洗液。整个标记过程可在１．５ｈ完成，全程标记率在６０％以上。测定了２１１Ａｔ－ＤＴＰＡ－ＩｇＧ在体外的稳定性，并通过比较标记物与Ｎａ２１１Ａｔ注射液在ＮＩＨ小鼠体内的分布和代谢，评价了２１１Ａｔ－ＤＴＰＡ－ＩｇＧ在体内的稳定性。
A method of labeling 211At human immunoglobulin G (IgG) by diethylenetriaminepentaacetic acid (DTPA) anhydride was studied. DTPA anhydride was synthesized and reacted with human IgG to prepare DTPA-IgG. Na211At solution with purified DTPA-IgG reaction at room temperature for 30min, SephadexG50 column separation and purification to give 211At-DTPA-IgG 0.1mol / LPBS eluent. The entire marking process can be completed in 1.5h, marking the entire rate of more than 60%. The stability of 211At-DTPA-IgG in vitro was determined and the in vivo stability of 211At-DTPA-IgG was evaluated by comparing the distribution and metabolism of the label with Na211At in NIH mice.