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建立一项简易的结肠直肠肿瘤的筛选检查方法。方法:采用亲合组织化学方法,用辣根过氧化酶(HRP)标记的花生凝集素(PNA)检测直肠粘液的T抗原。用肛指检查取得直肠粘液,涂于载玻片上。与HRP标记的PNA起反应,呈棕色者为阳性。结果:检测结肠直肠癌的敏感性为86.6％,特异性为92.5％。结论:直肠粘液T抗原检测筛选结肠直肠肿瘤是一种简易敏感的方法,较适合于人群普查。
Establish a simple screening test for colorectal tumors. METHODS: The T antigen of rectal mucus was detected by affinity-histochemical method using horseradish peroxidase (HRP)-labeled peanut lectin (PNA). The rectal mucus was obtained with an anal finger examination and applied on a slide. Reacts with HRP-labeled PNA and is brown positive. Results: The sensitivity of detecting colorectal cancer was 86.6% and the specificity was 92.5%. Conclusion: Detection of rectal mucin T antigen is a simple and sensitive method for screening colorectal neoplasms. It is more suitable for population screening.