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目的:探讨孔雀石绿(malachite green,MG)对小鼠睾丸基因组随机扩增多态性DNA(random amplified polymorphic DNA,RAPD)分子标记扩增图谱的影响。方法:24只成年雄性BALB/c小鼠随机均分为8组,MG浓度为:A1(0mg/L)、B1(100mg/L)、C1(400mg/L)、D1(800mg/L);A2(0mg/L)、B2(100mg/L)、C2(300mg/L)、D2(600mg/L)。A1～D1组连续经灌胃给药30d,A2～D2连续给药90d。取睾丸组织,提取睾丸基因组DNA,检测RAPD分子标记扩增图谱的基因组模板稳定性值(genometemplate stability,GTS)。结果:MG染毒各剂量组均可见睾丸基因组RAPD分子标记扩增图谱发生改变。MG染毒30d实验组中,GTS值B1组与A1组间无统计学差异(P>0.05),C1、D1组均显示低于A1组(P<0.05)。MG染毒90d,GTS值B2组与A2组间也无统计学差异(P>0.05),C2、D2组与A2组比较GTS值均显著降低(P<0.05)。D2组与D1组比较GTS值有显著降低(P<0.05)。GTS值与MG的剂量呈显著负相关(rs=-0.699,P<0.01)。结论:MG染毒能使小鼠睾丸GTS值降低,可能对睾丸基因组DNA造成损伤。
Objective: To investigate the effect of malachite green (MG) on the amplification of random amplified polymorphic DNA (RAPD) markers in mouse testis genome. Methods: Twenty - four adult male BALB / c mice were randomly divided into 8 groups. The concentrations of MG were A1 (0mg / L), B1 (400mg / L) A2 (0 mg / L), B2 (100 mg / L), C2 (300 mg / L) and D2 (600 mg / L). Groups A1 ~ D1 were given gavage continuously for 30 days and A2 ~ D2 for 90 days. Testicular tissue was taken, testis genomic DNA was extracted, and genome span stability (GTS) of RAPD molecular marker amplification was detected. Results: The amplifications of RAPD markers in testis genome were all observed in MG dose groups. There was no significant difference in the GTS value between B1 and A1 groups (P> 0.05). In C1 and D1 groups, the GTS value was lower than A1 group (P <0.05). There were no significant differences in GTS between groups B2 and A2 (P> 0.05). The GTS of C2 and D2 groups was significantly lower than that of A2 group (P <0.05). The GTS value of D2 group was significantly lower than that of D1 group (P <0.05). There was a significant negative correlation between GTS and MG dose (rs = -0.699, P <0.01). Conclusion: MG can decrease the testicular GTS in mice and may damage the testis DNA.