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目的探讨人内皮细胞特异性分子-1(ESM-1)在结直肠癌组织中的表达。方法原位杂交、免疫组织化学法检测ESM-1在9例正常黏膜、37例腺瘤和88例结直肠癌组织中的表达。结果原位杂交检测到ESM-1 mRNA在正常黏膜和腺瘤中的表达呈强阳性,表达率分别为67%和94%,而在结直肠癌组织中表达显著低于正常组织,为34%;免疫组化检测到正常黏膜和腺瘤中ESM-1蛋白表达率为67%和81%,而结直肠癌组织中表达率为39%。ESM-1mRNA和蛋白表达具有较好的一致性。结论ESM-1在结直肠癌组织中的表达明显降低。
Objective To investigate the expression of human endothelial cell-specific molecule-1 (ESM-1) in colorectal cancer. Methods In situ hybridization and immunohistochemistry were used to detect the expression of ESM-1 in 9 normal mucosa, 37 adenomas and 88 colorectal carcinomas. Results The expression of ESM-1 mRNA in normal mucosa and adenoma was strongly positive by in situ hybridization, the expression rates were 67% and 94% respectively, while it was significantly lower in colorectal cancer tissues than in normal tissues (34% Immunohistochemistry showed that ESM-1 protein expression was 67% and 81% in normal mucosa and adenoma, while the expression rate in ESCC was 39%. ESM-1 mRNA and protein expression with good consistency. Conclusion The expression of ESM-1 in colorectal cancer tissues was significantly decreased.