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分别采集海南省临高县3个地区的香蕉枯萎病发病土壤与健康土壤样品共6份,采用常规方法测定土壤理化性质,以末端限制性片段多态性分析(T-RFLP)技术研究不同地区发病与健康土壤微生物的多样性,并分析土壤微生物群落结构与土壤因子的关系.结果表明:同一地区发病蕉园土壤的大部分理化性质指标低于健康蕉园,以pH、有效P含量的差异最显著;T-RFLP结果表明供试蕉园发病土壤的细菌多样性比健康土壤丰富;3个地区的优势种分别为片段长度为144、147与233bp的T-RFs,通过系统发育分配工具比对,推断这3个地区的优势菌群可能属于枯草芽孢杆菌、葡萄球菌、反刍真杆菌;大部分T-RFs的分布与土壤碱解N、pH、速效K、有效P及含水量有关,且在发病土壤中的相对丰度大于健康土壤.
A total of 6 samples of banana wilt disease and healthy soil were collected from 3 areas in Lintao County, Hainan Province. Physical and chemical properties of soil were determined by conventional methods. T-RFLP The results showed that most of the physicochemical properties of banana soils in the same area were lower than that of healthy banana orchards. According to the difference of pH and effective P content, The most significant T-RFLP results showed that the bacterial diversity of tested banana plantations was more abundant than that of healthy soils. The dominant species in these three regions were T-RFs with fragment lengths of 144, 147 and 233 bp, respectively. On the other hand, it is concluded that the dominant bacteria in these three regions may belong to Bacillus subtilis, Staphylococcus, and Ruminobacter rhamnosus. The distribution of most T-RFs is related to soil alkaline hydrolysis N, pH, available K, available P and water content, and The relative abundance in diseased soil is greater than in healthy soil.