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目的 观察成纤维细胞在不同培养条件下生长 ,增殖 ,代谢及蛋白质合成等情况 ,探讨成纤维细胞的最佳培养模型 .方法 将正常皮肤中的成纤维细胞 (Ns Fb)分离出来并进行原代及继代培养 ,之后建立不同的成纤维细胞培养系统 ,分别利用绘制细胞生长曲线 ,3H-胸腺嘧啶 (3H- Td R)掺入及 3H-脯氨酸掺入等方法观察细胞的增殖 ,DNA代谢及胶原合成等情况 ,研究细胞在不同条件下的生物学特性 .结果 成纤维细胞在单层及纤维蛋白胶中培养时细胞的增殖 ,代谢及蛋白质合成等生物学功能均明显强于在两种胶原凝胶中培养时的情况 .结论 以纤维蛋白胶为支架的三维培养系统能更好地模拟生理条件下创面愈合时成纤维细胞的生物学特性 .
Objective To observe the growth, proliferation, metabolism and protein synthesis of fibroblasts under different culture conditions and to explore the best culture model of fibroblasts.Methods Normal skin fibroblasts (Ns Fb) were isolated and primary cultured And then subcultured. Then, different fibroblast culture systems were established. Cell growth curve, 3H-thymidine incorporation and 3H-proline incorporation were used to observe the cell proliferation, Metabolism and collagen synthesis, etc. The biological characteristics of cells under different conditions were studied.Results The biological functions of proliferation, metabolism and protein synthesis of fibroblasts in monolayer and fibrin glue were significantly stronger than those in two Collagen gels.Conclusion Fibrin glue-based three-dimensional culture system can better simulate the biological characteristics of fibroblasts when wound healing under physiological conditions.