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在室外中试规模的跑道池中,使用混凝沉淀处理后的沼液养殖高耐污钝顶螺旋藻,研究了螺旋藻的生长情况以及沼液中氮磷的去除情况,计算了沼液中氮磷向螺旋藻体的转化效率.在此基础上,结合小试研究,分析总结了使用沼液室外规模化养殖螺旋藻过程中存在的问题和对策.以12 d为一个培养周期,总共进行了6批次培养试验,其中3批次培养的螺旋藻浓度能够达到采收要求(D560>0.8);而另外3批次未能收获螺旋藻.成功的3个养殖批次中,螺旋藻采收后沼液中COD、氨氮、总氮、总磷分别减少了28.6%~48.5%、30.4%~48.5%、41.8%~48.6%、14.3%~94.5%;其中去除的总氮和总磷向螺旋藻细胞的转化率分别为12.1%~98.5%和21.2%~83.7%.沼液中的高浓度氨氮以及残存虫卵孵化产生的虫害是导致另外3批次培养螺旋藻生长缓慢的主要因素,使用生物处理技术降低沼液中氨氮含量、使用膜过滤技术去除沼液中虫卵对于稳定获得高产率的螺旋藻非常必要.
In the outdoor pilot-scale runway pond, the biogas slurry after coagulation and sedimentation treatment was used to breed high-resistant Spirulina platensis, the growth of Spirulina and the removal of nitrogen and phosphorus in the biogas slurry were studied. Nitrogen and phosphorus to Spirulina body transformation efficiency.On this basis, combined with a small pilot study, analysis and summary of the use of biogas slurry outdoor spirulina culture process problems and countermeasures to 12 d for a culture period, a total of Six batches of culture experiments were conducted, of which three batches of spirulina were able to reach the harvesting requirement (D560> 0.8), while the other three batches failed to harvest spirulina. The contents of COD, ammonia nitrogen, total nitrogen and total phosphorus decreased by 28.6% -48.5%, 30.4% -48.5%, 41.8% -48.6% and 14.3% -94.5% respectively after the post-harvest biogas slurry was collected. The removal of total nitrogen and total phosphorus The transformation rates of Spirulina platensis cells were 12.1% -98.5% and 21.2% -83.7%, respectively.The high concentrations of ammonia nitrogen in the biogas slurry and the insect pests caused by the hatching of the remaining eggs were the main factors causing the slow growth of Spirulina in the other three batches, Use biological treatment technology to reduce the ammonia nitrogen content in biogas slurry, use membrane filtration technology to remove biogas slurry Egg is necessary for stable and high yield of Spirulina.