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目的在基因转录水平探讨细胞凋亡相关途径对大鼠肝再生的作用。方法采用大鼠2/3部分肝切除(PH)方法,制备再生肝模型,同时设对照手术(假手术)。用查阅网站资料和相关论文等方法获得凋亡相关基因,用大鼠基因230 2.0芯片检测它们在大鼠再生肝中的表达情况,通过比较真、假手术中上述基因的表达差异性确定肝再生相关基因。结果细胞凋亡相关基因中,252个基因与肝再生相关。在肝再生启动(PH后0.5~4h)、G0/G1过渡(PH后4~6h)、细胞增殖(PH后6~66h)、细胞分化和结构功能重建期(PH后72~168h)等4个阶段起始表达的基因数为81、231、55和16,总表达的基因数为161、100、733和192,表明相关基因主要在肝再生启动阶段起始表达,在不同阶段发挥作用。它们共上调795次,下调291次,表明肝再生中大部分基因表达增强。它们的表达模式分为35种,表明肝再生中细胞凋亡相关基因的表达情况多样和复杂。结论15条细胞凋亡途径参与肝再生调控。
Objective To investigate the effect of apoptosis-related pathways on rat liver regeneration at gene transcriptional level. Methods Two-thirds partial hepatectomy (PH) was used to prepare regenerative liver model and control surgery (sham operation) was performed. Apoptosis-related genes were obtained by referring to website materials and related theses, and their gene expression was detected by rat gene 230 2.0 chip. The liver regeneration was confirmed by comparing the expression of the above genes in true and sham operation Related genes. Results Among the apoptosis related genes, 252 genes were related to liver regeneration. G0 / G1 transition (4 ~ 6h after PH), cell proliferation (6 ~ 66h after PH), cell differentiation and structural remodeling (72 ~ 168h after PH) The number of genes expressed at the beginning of each phase was 81, 231, 55 and 16, and the total number of genes expressed was 161, 100, 733 and 192, indicating that the related genes mainly start from initiation of liver regeneration and play roles in different stages. They were up-regulated 795 times, down 291 times, indicating that most of the gene expression in liver regeneration increased. Their expression patterns are divided into 35 types, indicating that the expression of apoptosis-related genes in liver regeneration is diverse and complex. Conclusion 15 apoptotic pathways are involved in the regulation of liver regeneration.