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目的在有免疫力的动物兔体内探索组织工程化软骨对软骨缺损的修复能力。方法软骨细胞种于经不同物质修饰的聚羟基乙酸支架体外培养后修复兔耳廓软骨缺损,与对照组比较,并从大体及组织学进行评价。结果软骨细胞种于经不同物质修饰的聚羟基乙酸支架体外培养后回植到兔耳廓软骨缺损部位,软骨缺损得到修复,但与正常软骨交界处为纤维组织。注射软骨细胞悬液、以聚羟基乙酸支架修复及空白对照组软骨缺损均未修复。结论软骨细胞种于经不同物质修饰的聚羟基乙酸支架体外培养后在有免疫力的动物兔体内可修复耳廓软骨缺损,但存在着界面愈合问题。
Objective To explore the ability of tissue-engineered cartilage to repair cartilage defects in an immunized rabbit. Methods Chondrocytes were cultured on polyglycolic acid scaffolds modified with different materials to repair the auricular cartilage defects in rabbits. Compared with the control group, the chondrocytes were evaluated both grossly and histologically. Results The chondrocytes were transplanted into the defect area of auricular cartilage in vitro after they were cultured with polyglycolic acid scaffold modified by different substances. The defect of cartilage was repaired, but the junction with normal cartilage was fibrous tissue. Injection of chondrocyte suspension, polyglycolic acid scaffold repair and blank control group were not repaired cartilage defects. Conclusion Chondrocytes can repair auricular cartilage defects in immune-bearing rabbits cultured in vitro with polyglycolic acid scaffold modified by different substances. However, there is a problem of interface healing.