将马铃薯Ｙ病毒普通系（ＰＶＹ０）的外壳蛋白基因克隆到表达质粒ｐＭＡＬｃ２中，构建这一基因在大肠杆菌中的表达载体ｐＭＡＬｃ２ＰＶＹ０ＣＰ。ＳＤＳＰＡＧＥ及Ｗｅｓｔｅｒｎｂｌｏｔｉｎｇ检测结果表明，这一表达栽体在Ｅ．ｃｏｌｉＤＨ５α中经ＩＰＴＧ诱导可表达分子量为７１．８ｋＤａ的特异性融合蛋白。以ａｍｙｌｏｓｅｒｅｓｉｎ亲合柱层析纯化这一融合蛋白为抗原，免疫家兔制备了效价为１∶１０２４的特异性抗血清。用该抗血清可通过对流免疫电泳、免疫双扩散及Ｗｅｓｔｅｒｎｂｌｏｔｉｎｇ对ＰＶＹ进行检测 The potato Y virus common line (PVY0) coat protein gene was cloned into the expression plasmid pMAL  c2, the construction of this gene in E. coli expression vector pMAL  c2  PVY0CP. SDS-PAGE and Western blotting results show that this expression vector in E. Induced by IPTG in E. coliDH5α, a specific fusion protein with a molecular weight of 71.8 kDa was expressed. The fusion protein was purified by amyloseresin affinity column chromatography and immunized rabbits to prepare a specific antiserum with titer 1:1024. The antiserum was used to detect PVY by convection-immune electrophoresis, double immunodiffusion and Western blotting