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采用RAPD技术构建了12个浙江主栽茭白品种的RAPD指纹图谱。利用河姆渡双季茭作为模板从50个随机引物中筛选出稳定且多态性高的7个引物用于茭白DNA的PCR扩增,共产生45条DNA片段,平均每个引物的扩增带为6.4条,其中15条扩增带具有遗传多样性。约占总DNA片段的33.3％。利用相似性聚类分析对PCR扩增产物进行二态性数据分析,结果表明,在相似系数2.1处可以将12个茭白品种分为5个聚类群,其中9个浙江本地品种间的遗传距离较近。初步结果也表明,单季茭与双季茭间的遗传差异比同一类型中茭白品种间的差异显著。
RAPD fingerprinting was used to construct 12 RAPD fingerprinting cultivars from Zhejiang Province. Using Hemudu double seasons as template, we screened out 50 random primers for stable and polymorphic 7 primers for PCR amplification of Baizhilu DNA, producing a total of 45 DNA fragments, the average amplification of each primer was 6.4, of which 15 amplified bands have genetic diversity. Accounting for 33.3% of the total DNA fragments. Similarity analysis was used to analyze the polymorphism of PCR products. The results showed that 12 polymorphic species could be divided into five clusters at a similarity coefficient of 2.1. Among them, the genetic distance between nine native Zhejiang breeds Closer. The preliminary results also showed that the genetic differences between Monochamus alternatus and Quercus mongolicus were significantly different than those of the same species.