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取第4代体外培养人牙周膜细胞(hPDLCs),分别与0、1、5、10、20ng/mL不同浓度TNF-α共同培养7d,采用MTT法测定培养后0～7d各时间点HPDLCs的增殖活力;用实时PCR检测不同浓度TNF-α刺激72h后细胞周期蛋白(CyclinD1)mRNA的表达;检测ALP活性。结果:与对照组相比,1、5ng/mL的TNF-α均可促进HPDLCs的增殖和Cy-clin mRNA和ALP的表达(P<0.05);10、20ng/mL浓度可抑制PDLCs的增殖和CyclinD1 mRNA、ALP的表达(P<0.05)。结论:不同浓度的TNF-α刺激牙周膜细胞后可以影响细胞的增殖和分化。
Human fourth generation of human periodontal ligament cells (hPDLCs) were cultured in vitro and incubated with different concentrations of TNF-α for 0, 1, 5, 10 and 20 ng / mL for 7 days. MTT assay was used to determine the percentage of HPDLCs . The expression of CyclinD1 mRNA was detected by real-time PCR at 72h after stimulation with different concentrations of TNF-α. The activity of ALP was detected. Results: Compared with the control group, 1,5ng / mL TNF-α could promote the proliferation of HPDLCs and the expression of Cy-clin mRNA and ALP (P <0.05); 10,20ng / mL could inhibit the proliferation of PDLCs and CyclinD1 mRNA and ALP expression (P <0.05). Conclusion: Different concentrations of TNF-α can stimulate the proliferation and differentiation of human periodontal ligament cells.