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目的:探讨急性创伤发生及恢复过程中外周血单个核细胞的端粒长度变化。方法:根据ISS创伤评分将2012年9月至2013年11月收治的74例30~50岁急性创伤病人,男49例,女25例,根据创伤严重度评分分成轻伤组、重伤组、极度重伤组,分别于创伤入院当天、术后当天及出院当天留取外周血,以及128例正常对照组采血,用定量PCR方法检测外周血单个核细胞的端粒长度,比较不同创伤程度病人的端粒长度在创伤发生和恢复过程中的差异。结果:在急性创伤入院当天,轻伤组的端粒长度(1.158±0.193)与对照组端粒长度(1.098±0.136)无统计学差异(P>0.05)。重伤组创伤当天的端粒长度(0.902±0.313)较对照组端粒长度缩短,有统计学差异(P<0.01)。极度重伤组创伤当天的端粒长度(0.460±0.118)较对照组端粒长度明显缩短,有显著统计学差异(P<0.001)。重伤组病人的端粒长度在创伤当天的离散度较大。重伤术后当天端粒长度较创伤入院当天延长(P<0.05),但与出院无差异。轻伤和重伤病人出院时的端粒长度和正常对照组之间无明显统计学差异。结论:急性创伤病人中,部分重伤和极度重伤病人的端粒长度在创伤当天较对照组缩短,在出院时趋于正常。
Objective: To investigate the changes of telomere length of peripheral blood mononuclear cells in the process of acute trauma and recovery. Methods: According to the ISS trauma score, 74 acute traumatic patients aged 30-50 years who were treated from September 2012 to November 2013 were 49 males and 25 females. According to the severity of trauma, 74 cases were divided into mild injury group, severe injury group, extreme degree group Severe injuries were collected on the day of trauma admission, on the day after surgery and on the day of discharge, and 128 normal controls. The telomere length of peripheral blood mononuclear cells was detected by quantitative PCR. Differences in grain length during wound healing and recovery. Results: There was no significant difference in telomere length (1.158 ± 0.193) and telomere length (1.098 ± 0.136) between the mild injury group and the control group on the day of admission for acute trauma (P> 0.05). The telomere length (0.902 ± 0.313) on the day of trauma in severe injury group was shorter than that in control group (P <0.01). The telomere length (0.460 ± 0.118) on the day of trauma in severely injured group was significantly shorter than that in control group (P <0.001). The length of telomere in severely injured patients was greater on the day of trauma. The telomere length on the day after severe injury was longer than that on the day of admission (P <0.05), but no difference with discharge. There was no statistically significant difference between the telomere length of patients with minor injuries and serious injuries discharged and the control group. Conclusion: In acute trauma patients, the length of telomere in some severely injured and severely injured patients was shorter than the control group on the day of trauma and tended to be normal at discharge.