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我们收集80例内镜活检胃粘膜组织标本,分析其 p16,APC 基因突变情况,结果如下.1 材料和方法1.1 材料免疫组化试剂购自北京中山生物技术有限公司;PCR 引物由北京赛百盛生物技术公司合成;随机引物购自北京泰克金生物技术有限公司;α-~(32)P-dCTP 购自北京亚辉生物医学工程公司;含有 p16 cDNA 序列的质粒 pBS 由北京医科大学张波教授惠赠;鲑鱼精 DNA,限制性内切酶购自华美生物技术公司.1.2 方法 p16免疫组化染色按常规进行.结果判定:阳性细胞数<25%为表达减少,无阳性细胞为表达缺失.p16斑点杂交及 Southern 杂交,按经典方法.p16 PCR-SSCP 分析参见文
We collected 80 endoscopic biopsies of gastric mucosal tissue samples and analyzed their p16, APC gene mutations. The results are as follows.1 Materials and methods 1.1 Materials Immunohistochemistry reagents were purchased from Beijing Zhongshan Biotechnology Co., Ltd.; PCR primers from Beijing赛Parkson Biotechnology Synthetic technology company; Random primers were purchased from Beijing Techkin Biotechnology Co., Ltd.; α-32P-dCTP was purchased from Beijing Yahui Biomedical Engineering Company; plasmid pBS containing p16 cDNA sequence was gifted by Prof. Zhang Bo from Beijing Medical University; Salmon sperm DNA, restriction enzyme was purchased from Sino Biotechnology Co., Ltd. 1.2 Methods p16 immunohistochemical staining was performed routinely. The results were judged as: the number of positive cells <25% was decreased, and no positive cells were lack of expression. p16 dot hybridization And Southern hybridization, according to classical methods. p16 PCR-SSCP analysis see text