目的探讨p16抑癌基因外显子 2缺失、突变与胃癌发生发展及组织学类型的关系。方法采用聚合酶链反应(PCR)、聚合酶链反应—单链构象多态性 (PCR -SSCP)分析分别检测胃癌中 p16基因外显子 2的缺失及突变。结果5 5例胃癌样本中PCR扩增 ,8例无扩增产物 ,2例产物明显减少 ,10例均可能为p16基因缺失 ,缺失率为 18.18%(10 /5 5 ) ,其余 45例胃癌、癌旁及正常胃组织均有PCR扩增产物出现 ;但所有胃癌标本SSCP分析均未见异常泳动带 ,无 p16基因突变。结论p16基因外显子 2缺失可能与胃癌发生发展有关 ;而 p16基因突变可能与胃癌发生无关  Objective  To investigate the relationship between the exon 2 deletion and mutation of p16 suppressor gene and the development and histology of gastric carcinoma. Methods 缺Detection and mutation of exon 2 of p16 gene in gastric cancer were detected by polymerase chain reaction (PCR) and polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP). Results 5 In 5 of 5 gastric cancer samples, no amplification products were found in 8 cases, and 2 cases of products were significantly reduced. All 10 cases were likely to have deletion of p16 gene, with a deletion rate of 18.18% (10/55), and the remaining 45 cases There were PCR amplification products in gastric cancer, paracancer and normal gastric tissues. However, no abnormal bands were detected in SSCP analysis of all gastric cancer specimens, and no mutation of p16 gene was found.  Conclusion 缺p16 gene exon 2 deletion may be related to the occurrence and development of gastric cancer; and p16 gene mutation may not be related to gastric cancer