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目的：探讨国人胃癌和结、直肠癌的ＡＰＣ基因突变情况。方法：采用ＰＣＲ－ＳＳＣＰ银染技术，分析了１４例胃癌和１４例散发性结、直肠癌的ＡＰＣ基因第１５外显子第６段和第７段的突变情况。结果：１４份结直肠癌原发灶样品中有１份ＡＰＣ基因第１５外显子第７段出现异常电泳条带，而１４份胃癌及其６份胃癌转移性淋巴结样品中却未观察到ＡＰＣ基因的异常电泳条带。结论：本组胃癌及淋巴结转移灶中未发现ＡＰＣ基因突变，而结、直肠癌的ＡＰＣ基因突变率为７％。
Objective: To investigate the mutation of APC gene in gastric cancer and colorectal cancer in Chinese. Methods: The PCR-SSCP silver staining technique was used to analyze the mutations of the 6th and 7th exon 15 of APC gene in 14 cases of gastric cancer and 14 cases of sporadic colorectal cancer. RESULTS: Abnormal electrophoresis bands were found in 1 out of 14 primary colorectal cancer samples in the 7th exon of APC gene, but no APC was observed in 14 gastric cancer and 6 gastric cancer metastatic lymph node samples. Abnormal electrophoretic bands of the gene. Conclusion: There is no APC gene mutation in gastric cancer and lymph node metastasis in this group. The mutation rate of APC gene in colorectal cancer is 7%.