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应用计算机分析了抗HPV16E7-ribozyme的体外切割反应结果,发现ribozyme和靶RNA的二级结构及其能量变化能影响ribozyme的切割活性。根据锤头结构ribozyme的能量变化模型,对抗HPV16E7-ribozyme和靶RNA在痘苗表达体系及稳定表达体系中的相互作用,进行计算机模拟分析。结果表明,计算机分析ribozyme和靶RNA在细胞内的相互作用,可以指导ribozyme的设计和在体内的应用研究。
The in vitro cleavage reaction of anti-HPV16E7-ribozyme was analyzed by computer and the secondary structure of ribozyme and target RNA and its energy change were found to affect the cleavage activity of ribozyme. According to the energy variation model of ribozyme, the interaction between HPV16E7-ribozyme and target RNA in vaccinia vaccine expression system and stable expression system was analyzed by computer simulation. The results show that computer analysis of ribozyme and target RNA interactions in the cell, can guide the ribozyme design and in vivo application.