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目的探讨Src激酶在D1/D5受体激动剂诱导的脊髓长时程增强(LTP)中的作用。方法细胞外记录技术在脊髓腰膨大部记录背角浅层神经元C-纤维诱发电位。结果①D1/D5受体激动剂SKF38393(250μmol/L)诱导的脊髓LTP的效应可被D1/D5受体拮抗剂SCH23390(20μmol/L)所阻断;②Src激酶的选择性抑制剂Genistein(200μmol/L)对脊髓背角C-纤维诱发电位的基础电位没有影响,但可阻断SKF诱导的脊髓背角LTP;③N-甲基-D-天冬氨酸受体(NMDAR)亚基2B特异性拮抗剂Ro25-6981(50μmol/L)阻断SKF诱导的脊髓背角LTP。结论脊髓背角Src激酶和NMDAR2B受体参与D1/D5受体诱导的脊髓LTP。
Objective To investigate the role of Src kinase in long-term potentiation (LTP) induced by D1 / D5 receptor agonist in spinal cord. Methods Extracellular recording technique was used to record the C-fiber evoked potentials of the superficial neurons in the dorsal horn of the spinal cord in the lumbar spine. Results ① The effect of Dl / D5 receptor agonist SKF38393 (250μmol / L) on the spinal cord LTP was blocked by the D1 / D5 receptor antagonist SCH23390 (20μmol / L); ② The selective inhibitor of Scrc kinase Genistein L) had no effect on the basal potential of C-fiber evoked potentials in spinal dorsal horn, but blocked the LTP of spinal dorsal horn induced by SKF; ③N-methyl-D-aspartate receptor (NMDAR) subunit 2B specificity Antagonist Ro25-6981 (50μmol / L) blocks SKF-induced LTP in spinal dorsal horn. Conclusion Spinal dorsal horn Src kinase and NMDAR2B receptor are involved in D1 / D5 receptor - induced spinal LTP.