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防御素(defensins)是一组具有抗微生物活性和细胞毒活性的多肽,相对分子质量3000～4000,包括人中性粒细胞多肽(human neutrophil peptides,HNP)。HNP占多形核中性粒细胞(polymorphonuclear neutrophils,PMNs)总蛋白/肽量的5％,含量4～6pg/PMN,目前在国内外文献中有关防御素对胃癌细胞的毒性作用研究未见报道。本研究目的在于观察HNP对胃癌细胞系的毒性作用,并探讨其毒性作用机制。 1 材料和方法 1.1 材料 人胃癌细胞系SGC-7901由军事医学科学院惠赠,小牛血清购自华美公司,RPMI1640培养基为Gibco产品,MTT及二甲基亚砜(DMSO)为Sigma产品,ELISA检测仪DG-3022
Defensins are a group of polypeptides with antimicrobial activity and cytotoxic activity, with a relative molecular mass of 3000-4000, including human neutrophil peptides (HNP). HNP accounted for 5% of the total protein/peptide amount of polymorphonuclear neutrophils (PMNs) and contained 4 to 6 pg/PMN. No studies have been reported on the toxic effects of defensins on gastric cancer cells in the literature at home and abroad. . The purpose of this study was to observe the toxic effects of HNP on gastric cancer cell lines and to explore its toxic mechanism. 1 Materials and Methods 1.1 Materials Human gastric cancer cell line SGC-7901 was kindly provided by the Academy of Military Medical Sciences, calf serum was purchased from Huamei Corporation, RPMI1640 medium was Gibco product, MTT and dimethyl sulfoxide (DMSO) was Sigma product, and ELISA was performed. DG-3022