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非侵入性快速胎儿核型分析需从孕妇外周血中分离胎儿细胞和用荧光原位杂交检测染色体。用特异性单克隆抗体与磁珠交联,结合梯度离心和微磁激活细胞分离法从孕妇外周血中富集到滋养细胞和/或胎儿有核红细胞以诊断胎儿异常。 研究对象为孕10～14周行绒毛活检的孕妇41例,每例取肝素化静脉血15～20ml,离心后弃血清,加入无血清培养液,再加入与磁珠交联的可识别滋
Noninvasive rapid fetal karyotyping requires the isolation of fetal cells from the peripheral blood of pregnant women and the detection of chromosomes by fluorescence in situ hybridization. Crosslinking with magnetic beads using specific monoclonal antibodies, the combination of gradient centrifugation and micro-magnetically activated cell isolation enrichment of peripheral blood from pregnant women to trophoblastic and / or fetal nucleated erythrocytes to diagnose fetal abnormalities. The study included 41 pregnant women who underwent villus biopsy from 10 to 14 weeks of gestation, 15 to 20 ml of heparinized venous blood per each case, serums were centrifuged, serum-free medium was added, and identifiable zygosaccharides