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延缓水稻叶片衰老,有增产的显著效果。因之,阐明衰老机理,对调节叶片早衰,进一步提高水稻产量有重要意义。 蛋白质降解是叶片衰老的基本特征。水稻叶片存在着三种以上蛋白水解酶,但在蛋白质降解中起主要作用的是巯基蛋白酶。 血红蛋白水解酶和羧肽酶的最适pH均为5,最适温度分别为55℃及45℃。硫酸铵分段盐析结果,血红蛋白水解酶和羧肽酶均在30—60％饱和度(0—4℃)沉淀的蛋白质中。
Delayed senescence of rice leaves, a significant yield increase. Therefore, to clarify the mechanism of aging, regulating leaf premature aging, and further improve the yield of rice is of great significance. Protein degradation is the basic characteristic of leaf senescence. There are three or more proteolytic enzymes in rice leaves, but thiol proteases play a major role in protein degradation. The optimum pH of hemoglobin and carboxypeptidase were 5, the optimum temperature was 55 ℃ and 45 ℃ respectively. As a result of the ammonium sulfate fractional salting-out, the hemoglobin and carboxypeptidase are both in the protein precipitated at 30-60% saturation (0-4 ° C).