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利用~(60)Coγ射线0.5—4.0kR 不同照射量辐照小麦“原冬一号”、“原冬94”、“农大139”雄配子发育不同时期的植株和用0.5—1.0kR 三个照射量、0.3-0.4%EMS 两个浓度处理“农大139”授粉后7-13小时的休眠合子。处理当代的结实率随照射量增加而下降;M_1代损伤效应明显,出苗率减少,植株生长受抑,抽穗延迟,育性降低;M_2代有较高的突变率,处理雄配子、合子总突变频率达17.1%,有益突变频率分别为4.8%、12.5%,并且变异谱较宽。γ射线处理合子的诱变效果优于 EMS。γ射线辐照雄配子的适宜时期为二核期、三核期,适宜剂量二核期为0.5-1.5kR;三核期为1.0-2.0kR:合子处理的适宜剂量γ射线为0.5-0.75kR,EMS 为0.3-0.4%。
The radiation of wheat at different levels of ~ (60) Co γ-rays at 0.5-4.0kR was used to irradiate the male gametes of “Dong Dong One”, “Yuan Dong 94” and “Nong Da 139” -1.0kR three exposure, 0.3-0.4% EMS two concentration treatment “Nongda 139 ” dormant zygotes after 7-13 hours of pollination. The treatment of contemporary seed setting rate decreased with the increase of irradiation dose; M_1 generation had obvious damage effect, reduced emergence rate, inhibited plant growth, delayed heading and fertility; M_2 generation had higher mutation rate, The frequency was 17.1%. The frequency of beneficial mutation was 4.8% and 12.5% respectively, and the variation spectrum was wider. γ-ray treatment zygote mutagenic effect is better than EMS. γ-ray irradiation of male gametes for the appropriate period of the two nuclear, trinuclear phase, the appropriate dose of dinucleate 0.5-1.5kR; Trinuclear phase of 1.0-2.0kR: zygotic suitable doses of γ-ray 0.5-0.75 kR , EMS is 0.3-0.4%.