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本文评述了4种测量微生物生物量方法之间的相关性。这4种方法是微生物体积法,氯仿熏蒸法(CO_2-C激增法),基质诱发呼吸法(SIR)及ATP含量法。土壤为分别经贮藏、风干及加葡萄糖处理的三种新西兰土壤。结果表明,各种方法之间都呈显著的正相关,r=0.69-0.88。其中CO_2-C激增法与ATP含量法之间的相关性最佳,与微生物体积法之间的相关性最差。应该采用2—3种方法来定量测定土壤中微生物的数量,特别应当重视使用未换算成生物量碳的数据来表示土壤内及土壤间的相对差异。因为换算成生物量碳的数据的误差很大。
This article reviews the correlation between four methods of measuring microbial biomass. These four methods are microbial volumetric method, chloroform fumigation (CO 2-C surge method), matrix-induced respiration (SIR) and ATP content method. The soils were three New Zealand soils that were stored, air-dried and added with glucose. The results showed that there was a significant positive correlation between various methods, r = 0.69-0.88. Among them, the correlation between CO_2-C surge method and ATP content method was the best, and the correlation with the microbial volume method was the worst. Two or three methods should be used to quantify the amount of microorganisms in the soil. In particular, the use of data not converted into biomass carbon should indicate the relative differences in soil and soil. Because of the large error in the data converted to biomass carbon.